Puromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5

• For generating drug-resistant feeder layers for the culture and selection of transfected undifferentiated ES and iPS cells
• 產品描述
• MEFs can be used as feeder cells for the maintenance of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in the undifferentiated state while under selection for a hygromycin-resistance marker that has been successfully incorporated into the ES cells or iPS cells. The cells must be mitotically inactivated by irradiation or mitomycin C treatment prior to use as feeder layers. MEFs are resistant to at least 140 μg/mL hygromycin.

Subtype:

凍存

Cell Type:

小鼠胚胎成纖維細胞; 多能干細胞

Species:

小鼠

Cell and Tissue Source:

其他

Donor Status:

正常

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