重組人PD-1蛋白(His)(Recombinant Human PD-1 Protein(His)),GMP級
產品貨號:GMP-TL756
產品描述(Description)
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
分子別名(Synonym)
PDCD1、D279、SLEB2
表達宿主(Expression host)
HEK293細胞
來源(Source)
Human
蛋白序列(Protein Sequence)
DNA 序列編碼人 PD-1( NCBI Reference Sequence: NP_005009.2)表達帶有 His 標簽在 C 末端
分子量(Molecular Weight)
重組人 PD-1 蛋白包含 148 個氨基酸,預測理論分子量為 21.16kD
純度(Purity)
≥95%采用SDS-PAGE凝膠和高效液相色譜分析
內毒素(Endotoxin)
<0.1EU/μg
生物活性(Biological Activity)
固定人 PD-1(His 標簽)0.5ug/mL (100uL/孔) 能夠結合人 PD-L1(Fc 標簽) 的線性范圍是 0.5-10ug/mL
穩定性(Stability)
凍干的樣本的可以在4℃保存24個月,溶解后的液體可以于-20℃保存6-12個月,并且避免反復凍融
組成(Component)
含有6%甘露醇的無菌PBS(PH 7.4)的凍干粉
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